Mind bomb-1 in dendritic cells is specifically required for Notch-mediated T helper type 2 differentiation.

Mind bomb-1 in dendritic cells is specifically required for Notch-mediated T helper type 2 differentiation.

Blog Article

In dendritic cell (DC)-CD4(+) T cell interaction, Notch signaling has been implicated in the CD4(+) T WASH BRUSH cell activation, proliferation, and subset differentiation.However, there has been a lot of debate on the exact role of Notch signaling.Here, we observed that expression of Mind bomb-1 (Mib1), a critical regulator of Notch ligands for the activation of Notch signaling, increases gradually as precursor cells differentiate into DCs in mice.

To clarify the role of Mib1 in DC-CD4(+) T cell interactions, we generated Mib1-null bone marrow-derived DCs.These cells readily expressed Notch ligands but failed to initiate Notch activation in the adjacent cells.Nevertheless, Mib1-null DCs were able to prime the activation and proliferation of CD4(+) T cells, suggesting that Notch activation in CD4(+) T cells is not required for these processes.

Intriguingly, stimulation of CD4(+) T cells with Mib1-null DCs resulted in dramatically diminished Th2 cell populations, while preserving Th1 cell populations, both in vitro and in vivo.Our results demonstrate that Mib1 in DCs Mountaineering - Accessoires - Fixations is critical for the activation of Notch signaling in CD4(+) T cells, and Notch signaling reinforces Th2 differentiation, but is not required for the activation or proliferation of the CD4(+) T cells.